Phage Display and Selections on Cells.

Phage Display and Selections on Cells.
Methods Mol Biol. 2018;1701:321-330
Authors: Fahr W, Frenzel A
Abstract
Antibody identification by phage display on protein or peptide targets is well established and many protocols are available. But there are many targets that cannot be expressed recombinantly or, like peptides, do not reflect correct folding of the protein. Most of these targets are cell surface receptors. Here, we describe a protocol for a panning strategy on cells to obtain specific binders to cell surface receptors. A depletion step is included to prevent enrichment of antibodies that bind to unwanted targets. Each step of the protocol is explained and variations of this protocol are given.
PMID: 29116513 [PubMed - in process]

Source: Mol Biol Cell - November 10, 2017 Category: Molecular Biology Authors: Fahr W, Frenzel A Tags: Methods Mol Biol Source Type: research