Epigenetic regulation of left-right asymmetry by DNA methylation
DNA methylation is a major epigenetic modification; however, the precise role of DNA methylation in vertebrate development is still not fully understood. Here, we show that DNA methylation is essential for the establishment of the left–right (LR) asymmetric body plan during vertebrate embryogenesis. Perturbation of DNA methylation by depletion of DNA methyltransferase 1 (dnmt1) or dnmt3bb.1 in zebrafish embryos leads to defects in dorsal forerunner cell (DFC) specification or collective migration, laterality organ malformation, and disruption of LR patterning. Knockdown of dnmt1 in Xenopus embryos also causes similar defects. Mechanistically, loss of dnmt1 function induces hypomethylation of the lefty2 gene enhancer and promotes lefty2 expression, which consequently represses Nodal signaling in zebrafish embryos. We also show that Dnmt3bb.1 regulates collective DFC migration through cadherin 1 (Cdh1). Taken together, our data uncover dynamic DNA methylation as an epigenetic mechanism to control LR determination during early embryogenesis in vertebrates.
Obituary A Remembrance of Juan "Jay" Rodrigo MD (1943-2018). Clin Orthop Relat Res. 2019 Jul;477(7):1760-1761 Authors: Calabro A PMID: 31210645 [PubMed - in process]
Abstract BACKGROUND: Above-knee amputation (AKA) is a severe but rare complication of TKA. Recent evidence suggests there are sex and racial disparities with regard to AKA after TKA. However, whether lower socioeconomic status is associated with an increased risk of AKA after TKA has not been conclusively established. QUESTIONS/PURPOSES: (1) Is low socioeconomic status or use of public health insurance plans associated with an increased risk of AKA after periprosthetic joint infection (PJI) of the knee? (2) Is race or sex associated with an increased risk of AKA after PJI of the knee? METHODS: This cross...
Commonwealth Fund–supported researchers examined how insurance coverage and employment changed in the early aftermath of Arkansas’s Medicaid work requirements, reporting that in the first six months, the requirements resulted in a loss of health care coverage and failed to increase employment rates.
This year, 2019, has become a critical time for abortion rights, with an unprecedented surge of abortion bans sweeping across the United States.
Introduction Parental barriers are a key factor to determine physical activity behaviors, such as active commuting to school in young people. The purpose of this study was to test the reliability and validity of a questionnaire which addresses parental ba...
In Cambodia, motorcycle use has spread rapidly in recent years, and serious accidents involving motorcycles have increased. Motorcycle accidents involving young people are particularly common, and there are various issues concerning traffic safety. To unde...
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