CRISPR/Cas9 Technology –Based Xenograft Tumors as Candidate Reference Materials for Multiple EML4-ALK Rearrangements Testing

The echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (ALK) receptor tyrosine kinase (EML4-ALK) rearrangement is an important biomarker that plays a pivotal role in therapeutic decision making for non –small-cell lung cancer (NSCLC) patients. Ensuring accuracy and reproducibility of EML4-ALK testing by fluorescence in situ hybridization, immunohistochemistry, RT-PCR, and next-generation sequencing requires reliable reference materials for monitoring assay sensitivity and specificity.

Source: Journal of Molecular Diagnostics - July 18, 2017 Category: Pathology Authors: Rongxue Peng, Rui Zhang, Guigao Lin, Xin Yang, Ziyang Li, Kuo Zhang, Jiawei Zhang, Jinming Li Tags: Regular Article Source Type: research