Transcriptome analysis of distinct long non-coding RNA transcriptional fingerprints in lung adenocarcinoma and squamous cell carcinoma

In this study, we tried to develop and validate novel long non-coding RNA (lncRNA)-based signatures to facilitate the diagnosis and individualized treatment of LUAD and LUSC. To analyze the distinct lncRNA profiling in LUAD and LUSC, the paired tissue samples of RNA sequencing or microarray data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) was used. We found that lncRNA expression pattern is different in LUAD and LUSC, and three up-regulated and down-regulated lncRNAs expressions in LUAD and LUSC tissues were validated. In addition, knockdown of the up-regulated lncRNA AFAP1-AS1 and LINC00511 impaired LUAD cell proliferation, while knockdown of PVT1 inhibited LUSC cell growth. Furthermore, four 6-lncRNAs signature expression patterns were found to be significantly associated with LUAD and LUSC patient overall and progression-free survival. Taken together, the different lncRNAs signature might accurately distinguish LUAD and LUSC and predict clinical outcome for these two different subtypes of NSCLC.
Source: Tumor Biology - Category: Cancer & Oncology Source Type: research