Characterization of Mosaicism in Different Easy-to-Acquire Body Tissues Such As Buccal Smears, Skin Abrasions, Hair Root Cells, or Urine

Mosaic karyotypes are present in at least 0.3 to 1 % of clinical cases analyzed by banding cytogenetics. It is well known that the pattern of mosaicism can be extremely variant in different tissue types of the same patient. However, normally in maximum two different tissues of a child or an adult are cytogenetically studied, i.e., peripheral blood and skin fibroblasts. Here preparation protocols for four easily acquirable further tissues are presented. The resulting preparations of interphase nuclei can be used in routine interphase FISH applications. Here it is summarized how to treat buccal smears, skin abrasions, hair root cells, or urine before the FISH procedure.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news