Application of FISH to Previously GTG-Banded and/or Embedded Cytogenetic Slides

GTG-banded slides are often considered to be worthless for further molecular cytogenetic analyses. In this chapter we describe a simple protocol on how to reactivate such GTG-banded slides for further use in molecular cytogenetics. The slides can be previously Eukitt® or“Canada Balsam” embedded or previously not covered with a coverslip at all. With this possibility at hand, archival material of rare cytogenetic aberrations as well as more profound studies on actual cases with limited access to material can be performed.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news