One-Day Quick FISH

The classical FISH approach requires overnight incubation for proper hybridization result. Tissue morphological features are varying due to aggressive pretreatment and high temperatures applied. To increase the speed of the molecular cytogenetic finding and eliminate some of the technical limitations, an alternative one-day FISH method was recently introduced. This procedure allows the completion and evaluation of a FISH reaction within one day by the reduction of the hybridization time to 60–120 min. Moreover, the low denaturation temperature significantly contributes to better tissue and cell morphology of the patient samples. The utility of the instant quality FISH (IQFISH) was carefully evaluated for the determination of HER2 copy number status in breast carcinoma and ALK translocation status in lung adenocarcinoma samples in a clinical setting. In summary, the one-day IQFISH diagnostic kits point with fast and stable hybridization reaction in the diagnostic practice without any major loss compared to the conventional (overnight) FISH procedure.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news