Homemade Locus-Specific FISH Probes: Bacterial Artificial Chromosomes

Besides the well-known applications of bacterial artificial chromosomes (BACs) in classical molecular genetics, these probes are also well suited for molecular cytogenetic studies. BACs are nowadays the most often applied locus-specific probes in FISH. Various applications are possible like gene mapping, FISH banding, determination of chromosomal breakpoints, characterization of derivative chromosomes, studies on the interphase architecture, or the karyotypic evolution. Here the basic principle how BACs can be hybridized in situ on chromosome preparations is outlined. Moreover, an overview is given on possible questions to be studied using BACs as FISH probes.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news