Effect of ionophore salinomycin on the antibiotic resistance in Clostridium difficile detected in mice

AbstractIn the present study, an in vivo model was established to pursue the possible influence of ionophore, salinomycin, on the mobile resistance genes inClostridium difficile. Fecal samples of 48 healthy adult mice were initially checked for the presence ofC. difficile using a species-specific pair of primers. The animals were subsequently divided into the following experimental groups as follows salinomycin + tetracycline, tetracycline, salinomycin, and control (no treatment), for 10  days. Fecal sample was then collected from each group at days 0, 3, and 5 posttreatment, anaerobically enriched in the thioglycolate broth followed by sub-culturing onto blood agar plates. The growth curve and minimum inhibitory concentration (MIC) of groups were then recorded. A PCR assay was als o conducted to amplifytet A andtet M genes corresponding to tetracycline. Results showed that salinomycin likely influenced the mobile resistance gene in gram-positive bacteria. Moreover, it may reduce the natural resistance of gram-positive bacteria and also inhibit the transfer of tetracycline mobile resistance gene, in vivo.

Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research