Surface-layer protein from Caulobacter crescentus: expression, purification and X-ray crystallographic analysis

Protein surface layers are self-assembling, paracrystalline lattices on the surface of many prokaryotes. Surface-layer proteins have not benefited from widespread structural analysis owing to their resistance to crystallization. Here, the successful expression of a truncated version of RsaA, the surface-layer protein from Caulobacter crescentus, from a Caulobacter protein-expression system is reported. The purification, crystallization and initial X-ray diffraction analysis of the truncated RsaA, the largest surface-layer protein studied to date and the first from a Gram-negative bacterium, are also reported.

Source: Acta Crystallographica Section F - August 8, 2016 Category: Biochemistry Authors: Jones, M.D. Chan, A.C.K. Nomellini, J.F. Murphy, M.E.P. Smit, J. Tags: S-layer surface-layer protein RTX motif phasing Gram-negative research communications Source Type: research