Clinical Evaluation of Rapid Diagnostic Test Kit for Scrub Typhus with Improved Performance.

Clinical Evaluation of Rapid Diagnostic Test Kit for Scrub Typhus with Improved Performance. J Korean Med Sci. 2016 Aug;31(8):1190-6 Authors: Kim YJ, Park S, Premaratna R, Selvaraj S, Park SJ, Kim S, Kim D, Kim MS, Shin DH, Choi KC, Kwon SH, Seo W, Lee NT, Kim SH, Kang HK, Kim YW Abstract Diagnosis of scrub typhus is challenging due to its more than twenty serotypes and the similar clinical symptoms with other acute febrile illnesses including leptospirosis, murine typhus and hemorrhagic fever with renal syndrome. Accuracy and rapidity of a diagnostic test to Orientia tsutsugamushi is an important step to diagnose this disease. To discriminate scrub typhus from other diseases, the improved ImmuneMed Scrub Typhus Rapid Diagnostic Test (RDT) was evaluated in Korea and Sri Lanka. The sensitivity at the base of each IgM and IgG indirect immunofluorescent assay (IFA) in Korean patients was 98.6% and 97.1%, and the specificity was 98.2% and 97.7% respectively. The sensitivity and specificity for retrospective diagnosis at the base of IFA in Sri Lanka was 92.1% and 96.1%. ImmuneMed RDT was not reactive to any serum from seventeen diseases including hemorrhagic fever with renal syndrome (n = 48), leptospirosis (n = 23), and murine typhus (n = 48). ImmuneMed RDT shows superior sensitivity (98.6% and 97.1%) compared with SD Bioline RDT (84.4% at IgM and 83.3% at IgG) in Korea. The retrospective diagnosis of ImmuneMed RDT exhibits 94.0% identit...
Source: J Korean Med Sci - Category: Journals (General) Authors: Tags: J Korean Med Sci Source Type: research