Measurement of peroxiredoxin activity.
Measurement of peroxiredoxin activity.
Curr Protoc Toxicol. 2011 Aug;Chapter 7:Unit7.10
Authors: Nelson KJ, Parsonage D
Abstract
Peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. Protocols are provided to measure Prx activity with peroxide by (1) a coupled reaction with NADPH, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the FOX assay.
PMID: 21818753 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Toxicology - Category: Toxicology Tags: Curr Protoc Toxicol Source Type: research
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