From bottom-up to cell surface proteomics: detergents or no detergents, that is the question

Biochem Soc Trans. 2024 Apr 26:BST20231020. doi: 10.1042/BST20231020. Online ahead of print.ABSTRACTMeasuring the expression levels of membrane proteins (MPs) is crucial for understanding cell differentiation and tissue specificity, defining disease characteristics, identifying biomarkers, and developing therapeutics. While bottom-up proteomics addresses the need for accurately surveying the membrane proteome, the lower abundance and hydrophobic nature of MPs pose challenges in sample preparation. As MPs normally reside in the lipid bilayer, conventional extraction methods rely on detergents, introducing here a paradox - detergents prevent aggregation and facilitate protein processing, but themselves become contaminants that interfere with downstream analytical applications. Various detergent removal methods exist to mitigate this issue, including filter-aided sample preparation, SP3, suspension trapping, and membrane mimetics. This review delves into the fundamentals of each strategy, applications, merits, and limitations, providing insights into their effectiveness in MP research.PMID:38666604 | DOI:10.1042/BST20231020

Source: Biochemical Society Transactions - April 26, 2024 Category: Biochemistry Authors: Zora Brough Zhiyu Zhao Franck Duong van Hoa Source Type: research