A cost-effective and labor-saving method for detecting HLA-B27 status via sequence-encoded fluorescence amplification assay
Identification of Human leukocyte antigen B27 (HLA-B27) by flow cytometry (FCM) has been widely applied in clinical practice for auxiliary diagnosis of ankylosing spondylitis (AS). However, FCM requires fresh-prepared samples and relies on expensive equipment, reagents, and experienced operator. To provide a cheaper and more convenient method for HLA-B27 detection, we proposed a new method termed sequence-encoded fluorescence amplification assay (SEFA), which specially recognized sequences of HLA-B27 gene (HLA-B*27) covering current common subtypes in a single closed tube.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Nan Sheng, Li Zhao, Shuyun Pang, Wenwen Wang, Panfeng Feng, Jing Zhao, Xiaoxiang Chen, Yingying Gao Tags: Regular Article Source Type: research