Covalent activity-based probes for imaging of serine proteases

Biochem Soc Trans. 2024 Apr 17:BST20231450. doi: 10.1042/BST20231450. Online ahead of print.ABSTRACTSerine proteases are one of the largest mechanistic classes of proteases. They regulate a plethora of biochemical pathways inside and outside the cell. Aberrant serine protease activity leads to a wide variety of human diseases. Reagents to visualize these activities can be used to gain insight into the biological roles of serine proteases. Moreover, they may find future use for the detection of serine proteases as biomarkers. In this review, we discuss small molecule tools to image serine protease activity. Specifically, we outline different covalent activity-based probes and their selectivity against various serine protease targets. We also describe their application in several imaging methods.PMID:38629725 | DOI:10.1042/BST20231450

Source: Biochemical Society Transactions - April 17, 2024 Category: Biochemistry Authors: Marcin Skorenski Shanping Ji Steven H L Verhelst Source Type: research