DREAMweb: An online tool for graph ‐based modeling of NMR protein structure

AbstractThe value of accurate protein structural models closely conforming to the experimental data is indisputable. DREAMweb deploys an improved DREAM algorithm, DREAMv2, that incorporates a tighter bound in the constraint set of the underlying optimization approach. This reduces the artifacts while modeling the protein structure by solving the distance-geometry problem. DREAMv2 follows a bottom-up strategy of building smaller substructures for regions with a larger concentration of experimental bounds and consolidating them before modeling the rest of the protein structure. This improves secondary structure conformance in the final models consistent with experimental data. The proposed method efficiently models regions with sparse coverage of experimental data by reducing the possibility of artifacts compared to DREAM. To balance performance and accuracy, smaller substructures ( ∼200$\sim 200$ atoms) are solved in this regime, allowing faster builds for the other parts under relaxed conditions. DREAMweb is accessible as an internet resource. The improvements in results are showcased through benchmarks on 10 structures. DREAMv2 can be used in tandem with any NMR-based prot ein structure determination workflow, including an iterative framework where the NMR assignment for the NOESY spectra is incomplete or ambiguous. DREAMweb is freely available for public use athttp://pallab.cds.iisc.ac.in/DREAM/ and downloadable athttps://github.com/niladriranjandas/DREAMv2.git.

Source: Proteomics - April 17, 2024 Category: Biochemistry Authors: Niladri Ranajan Das, Kunal Narayan Chaudhury, Debnath Pal Tags: TECHNICAL BRIEF Source Type: research