ATP-Dependent Steps in Peroxisomal Protein Import

Annu Rev Biochem. 2024 Apr 15. doi: 10.1146/annurev-biochem-030222-111227. Online ahead of print.ABSTRACTPeroxisomes are organelles that play a central role in lipid metabolism and cellular redox homeostasis. The import of peroxisomal matrix proteins by peroxisomal targeting signal (PTS) receptors is an ATP-dependent mechanism. However, the energy-dependent steps do not occur early during the binding of the receptor-cargo complex to the membrane but late, because they are linked to the peroxisomal export complex for the release of the unloaded receptor. The first ATP-demanding step is the cysteine-dependent monoubiquitination of the PTS receptors, which is required for recognition by the AAA+ peroxins. They execute the second ATP-dependent step by extracting the ubiqitinated PTS receptors from the membrane for release back to the cytosol. After deubiquitination, the PTS receptors regain import competence and can facilitate further rounds of cargo import. Here, we give a general overview and discuss recent data regarding the ATP-dependent steps in peroxisome protein import.PMID:38621235 | DOI:10.1146/annurev-biochem-030222-111227

Source: Annual Review of Biochemistry - April 15, 2024 Category: Biochemistry Authors: Harald W Platta Julia Jeske Nadine Schmidt Ralf Erdmann Source Type: research