Molecular Machines that Facilitate Bacterial Outer Membrane Protein Biogenesis

Annu Rev Biochem. 2024 Apr 11. doi: 10.1146/annurev-biochem-030122-033754. Online ahead of print.ABSTRACTAlmost all outer membrane proteins (OMPs) in Gram-negative bacteria contain a β-barrel domain that spans the outer membrane (OM). To reach the OM, OMPs must be translocated across the inner membrane by the Sec machinery, transported across the crowded periplasmic space through the assistance of molecular chaperones, and finally assembled (folded and inserted into the OM) by the β-barrel assembly machine. In this review, we discuss how considerable new insights into the contributions of these factors to OMP biogenesis have emerged in recent years through the development of novel experimental, computational, and predictive methods. In addition, we describe recent evidence that molecular machines that were thought to function independently might interact to form dynamic intermembrane supercomplexes. Finally, we discuss new results that suggest that OMPs are inserted primarily near the middle of the cell and packed into supramolecular structures (OMP islands) that are distributed throughout the OM.PMID:38603556 | DOI:10.1146/annurev-biochem-030122-033754

Source: Annual Review of Biochemistry - April 11, 2024 Category: Biochemistry Authors: Matthew Thomas Doyle Harris D Bernstein Source Type: research