Development of polymorphic microsatellite markers for distylous-homostylous Primula secundiflora (Primulaceae) using HiSeq sequencing

Genes Genet Syst. 2024 Mar 29. doi: 10.1266/ggs.23-00340. Online ahead of print.ABSTRACTPrimula secundiflora is an insect-pollinated, perennial herb belonging to section Proliferae (Primulaceae) and exhibits considerable variation in mating system with predominantly outcrossing populations comprising long-styled and short-styled floral morphs and selfing populations comprising only homostyles. To facilitate future investigations of the population genetics and mating patterns of this species, we developed 25 microsatellite markers from P. secundiflora using next-generation sequencing and measured polymorphism and genetic diversity in a sample of 30 individuals from three natural populations. The markers displayed relatively high polymorphism, with the number of observed alleles per locus ranging from three to 16 (mean = 8.36). The observed and expected heterozygosities ranged from 0.100 to 1.000 and 0.145 to 0.843, respectively. Twenty-one of the loci were also successfully amplified in P. denticulata. These microsatellite markers could provide powerful tools for investigating patterns of population genetic diversity and the evolutionary relationships between distyly and homostyly in this species.PMID:38556272 | DOI:10.1266/ggs.23-00340

Source: Genes and Genetic Systems - March 31, 2024 Category: Genetics & Stem Cells Authors: Hua-Ying Sun Wen-Ping Zhang Wei Zhou Zhi-Kun Wu Lan-Ping Zheng Source Type: research