Light-triggered AND logic tetrapeptide dynamic covalent assembly

Colloids Surf B Biointerfaces. 2024 Apr 1;238:113885. doi: 10.1016/j.colsurfb.2024.113885. Online ahead of print.ABSTRACTWe demonstrate light-triggered dynamic covalent assembly of a linear short tetrapeptide containing two terminal cysteine residues in an AND logic manner. A photobase generator is introduced to accomplish light-mediated pH regulation to increase the reduction potential of thiols in the tetrapeptide, which activates its oxidative polymerization through disulfide bonds. Interestingly, it is elucidated that under light irradiation, mere co-existence of photobase generator and the oxidizing agent permits the polymerization performance of this tetrapeptide. Hence, a light-triggered AND logic dynamic covalent assembly of a tetrapeptide is achieved. Further, upon redox response, the reversible aggregation and disaggregation can be transformed for numerous times due to the dynamic covalent feature of disulfide bond. As a comparison, no assembly occurs for a short peptide containing one terminal cysteine residue under the same stimuli condition. This work offers a new approach to remotely control programmable molecular assembly of short linear peptides based on dynamic covalent bond, holding great potential in wide bioapplications.PMID:38574405 | DOI:10.1016/j.colsurfb.2024.113885

Source: Colloids and Surfaces - April 4, 2024 Category: Biotechnology Authors: Yuhua Tang Liang Gao Jinbo Fei Source Type: research