Substrate specificity of a ketosynthase domain involved in bacillaene biosynthesis

Abstract
An isotopic labelling method was developed to investigate substrate binding by ketosynthases, exemplified by the second ketosynthase of the polyketide synthase BaeJ involved in bacillaene biosynthesis (BaeJ-KS2). For this purpose, both enantiomers of a 13C-labelled N-acetylcysteamine thioester (SNAC ester) surrogate of the proposed natural intermediate of BaeJ-KS2 were synthesised, including an enzymatic step with glutamate decarboxylase, and incubated with BaeJ-KS2. Substrate binding was demonstrated through 13C NMR analysis of the products against the background of various control experiments.

Beilstein J. Org. Chem. 2024, 20, 734–740. doi:10.3762/bjoc.20.67

Source: Beilstein Journal of Organic Chemistry - April 5, 2024 Category: Chemistry Authors: Beilstein Journal of Organic Chemistry - Syndicated Feeds Tags: bacillaene biosynthesis enzyme mechanisms isotopes trans-AT polyketide synthases Letter Source Type: research