The significant contribution of comammox bacteria to nitrification in a constructed wetland revealed by DNA-based stable isotope probing

This study investigated the contributions of CMX and two canonical ammonia-oxidizing microorganisms, ammonia-oxidizing bacteria (AOB) and archaea to nitrification in four regions (sediment, shoreside, adjacent soil, and water) of a typical CW using DNA-based stable isotope probing. The results revealed that CMX not only widely occurred in sediment and shoreside zones with high abundance (5.08 × 104 and 6.57 × 104 copies g-1 soil, respectively), but also actively participated in ammonia oxidation, achieving ammonia oxidation rates of 1.43 and 2.00 times that of AOB in sediment and shoreside, respectively. Phylogenetic analysis indicated that N. nitrosa was the dominant and active CMX species. These findings uncovered the crucial role of CMX in nitrification of sediment and shoreside, providing a new insight into nitrogen cycle of constructed wetlands.PMID:38548031 | DOI:10.1016/j.biortech.2024.130637

Source: Bioresource Technology - March 28, 2024 Category: Biotechnology Authors: Anqi Zhang Mingyang Zhu Yize Zheng Zhichao Tian Guangli Mu Maosheng Zheng Source Type: research