Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Muthukumar et  al. use genome-wide and systematic arrayed CRISPRi screens to identify ⍺-helical OMM protein biogenesis and quality control factors that triage substrates by topology. They establish NAC as a targeting factor for polytopic proteins and identify TTC1 as a cytosolic chaperone for signal-anchored p roteins, using a C-terminal hydrophobic groove.

Source: Molecular Cell - February 29, 2024 Category: Cytology Authors: Gayathri Muthukumar, Taylor A. Stevens, Alison J. Inglis, Theodore K. Esantsi, Reuben A. Saunders, Fabian Schulte, Rebecca M. Voorhees, Alina Guna, Jonathan S. Weissman Tags: Article Source Type: research