Systematically amino acid analysis: Advancements in extinction coefficient determination for therapeutic proteins

Anal Biochem. 2024 Mar 15:115508. doi: 10.1016/j.ab.2024.115508. Online ahead of print.ABSTRACTBiologicals developers often face challenges in accurately determining the extinction coefficient (EC) measurement. We have successfully improved the precision and robustness of the widely recognized amino acid analysis method for EC determination, through a stepwise optimization process. Extensive analyses based on 114 observations, covering eight proteins over three years were performed, with a maximum relative standard deviation of 1.5% among multiple analysts, and a maximum deviation of 2.8% from the theoretical EC across the eight given proteins examined.PMID:38494101 | DOI:10.1016/j.ab.2024.115508

Source: Analytical Biochemistry - March 17, 2024 Category: Biochemistry Authors: Ya Xu Zhenzhen Shao Lei Zhang Yanjing Cao Zhuoyu Liu Zhongli Zhang Source Type: research