DNA reference reagents for genotyping RH variants

Patients who carry RH blood group variants may develop Rh alloantibodies requiring matched red cell transfusions. Serologic reagents for Rh variants often fail to specifically identify variant Rh antigens and are in limited supply. Therefore, red cell genotyping assays are essential for managing transfusions in patients with clinically relevant Rh variants. Well-characterized DNA reference reagents are needed to ensure quality and accuracy of the molecular tests. Eight lyophilized DNA reference reagents, representing 21 polymorphisms in RHD and RHCE, were produced from an existing repository of immortalized B-lymphoblastoid cell lines at CBER/U.S.

Source: Journal of Molecular Diagnostics - March 15, 2024 Category: Pathology Authors: Emilia Sippert, Evgeniya Volkova, Meagan Rippee-Brooks, Gregory A. Denomme, Willy A. Flegel, Christine Lee, Richardae Araojo, Orieji Illoh, Zhugong Liu, Maria Rios, Collaborative Study Group Tags: Regular Article Source Type: research