Structural basis for plasmid restriction by SMC JET nuclease

The JET nuclease (Wadjet) is a prokaryotic SMC-based defense system that exclusively cleaves plasmid DNA. Roisn é-Hamelin et al. determine structures of plasmid-borne JET, elucidating DNA bending/kinking prior to cleavage. Together with biochemical work, this supports a model of plasmid recognition by SMC motor stalling when circular DNA is completely extruded.

Source: Molecular Cell - February 2, 2024 Category: Cytology Authors: Florian Roisn é-Hamelin, Hon Wing Liu, Michael Taschner, Yan Li, Stephan Gruber Tags: Article Source Type: research