Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization

The structural and interactive properties of two novel hemifluorinated surfactants, F2H9-β-M and F4H5-β-M, the syntheses of which were based on the structure and hydrophobicity of the well known dodecyl-β-maltoside (DD-β-M), are described. The shape of their micellar assemblies was characterized by small-angle X-ray scattering and their intermicellar interactions in crystallizing conditions were measured by dynamic light scattering. Such information is essential for surfactant phase-diagram determination and membrane-protein crystallization.

Source: Acta Crystallographica Section F - June 27, 2015 Category: Biochemistry Authors: Dahani, M.Barret, L.-A.Raynal, S.Jungas, C.Pernot, P.Polidori, A.Bonneté, F. Tags: membrane protein surfactant micelle scattering techniques phase diagram crystallization research communications Source Type: research