Guidelines on Antibody Use for Physiology Research

Am J Physiol Renal Physiol. 2024 Jan 18. doi: 10.1152/ajprenal.00347.2023. Online ahead of print.ABSTRACTAntibodies are one of the most used reagents in scientific laboratories and are critical components for a multitude of experiments in physiology research. Over the last decade, concerns about many biological methods, including those that use antibodies, have arisen as many labs were unable to reproduce the scientific data obtained in other laboratories. While some of the lack of reproducibility may be due to inadequate reporting of detailed methods, the use of unvalidated antibodies was identified as a major source of results that lacked reproducibility. The goal of this guidelines article is to review current use and provide best practice guidelines on commonly used techniques involving antibodies, including immunoblotting, immunohistochemistry, and flow cytometry. Knowledge and use of these practices will increase the rigor and reproducibility of these techniques and elevate the quality of physiology research.PMID:38234298 | DOI:10.1152/ajprenal.00347.2023

Source: American Journal of Physiology. Renal Physiology - January 18, 2024 Category: Physiology Authors: Heddwen L Brooks Lisandra E de Castro Br ás Keith R Brunt Megan A Sylvester Michelle S Parvatiyar Padmini Sirish Shyam S Bansal Rasheed Sule Ashley L Eadie Mark A Knepper Robert A Fenton Merry L Lindsey Kristine Y DeLeon-Pennell Aldrin V Gomes Source Type: research