Ubiquiton —An inducible, linkage-specific polyubiquitylation tool

Analytical tools for investigating ubiquitin signaling are abundant, but methods to induce linkage-specific polyubiquitylation of a protein of interest have been missing. Renz et  al. have now developed rapamycin-inducible E3s specific for three major linkages and validated them for use in vitro in budding yeast and in mammalian cells.

Source: Molecular Cell - December 15, 2023 Category: Cytology Authors: Christian Renz, Evrydiki Asimaki, Cindy Meister, V éronique Albanèse, Kirill Petriukov, Nils C. Krapoth, Sabrina Wegmann, Hans-Peter Wollscheid, Ronald P. Wong, Amitkumar Fulzele, Jia-Xuan Chen, Sébastien Léon, Helle D. Ulrich Tags: Technology Source Type: research