Determination for KIR genotype and allele copy number via real-time quantitative PCR method

AbstractKiller cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) play crucial roles in regulating NK cell activity. Here, we report a real-time quantitative PCR (qPCR) to genotype allKIR genes and their copy numbers simultaneously. With 18 pairs of locus-specific primers, we identifiedKIR genes by Ct values and determinedKIR copy number using the 2−∆Ct method. Haplotypes were assigned based onKIR gene copy numbers. The real-time qPCR results were consistent with the NGS method, except for one sample withKIR2DL5 discrepancy. qPCR is a multiplex method that can identifyKIR copy number, which helps obtain a relatively accurate haplotype structure, facilitating increasedKIR research in laboratories where NGS or other high-resolution methods are not available.

Source: Immunogenetics - January 11, 2024 Category: Genetics & Stem Cells Source Type: research