cDNA Hybrid Capture Improves Transcriptome Analysis on Low-Input and Archived Samples

In this study we describe the combined use of RNA sequencing with an exome capture selection step to enhance the yield of on-exon sequencing read data when compared with RNA sequencing alone. In particular, the exome capture step preserves the dynamic range of expression, permitting differential comparisons and validation of expressed mutations from limited and FFPE preserved samples, while reducing the data generation requirement. We conclude that cDNA hybrid capture has the potential to significantly improve transcriptome analysis from low-yield FFPE material.

Source: The Journal of Molecular Diagnostics - June 19, 2015 Category: Pathology Source Type: research