FOXO3-activated HOTTIP Sequesters MiR-615-3p Away from COL2A1 to Mitigate Intervertebral Disc Degeneration

Knockout of FOXO3 was found to impair IVD maturation and homeostasis in postnatal mice as well as facilitating extracellular matrix (ECM) degradation. RNA sequencing can uncover disease-related gene expression and investigate disease pathophysiology. High-throughput transcriptome sequencing and experimental validations were used to identify the essential gene and mechanism involved in intervertebral disc degeneration (IDD). Nucleus pulposus (NP) tissue samples were collected from the mice with conditional knockout of FOXO3 (FOXO3 KO) for high-throughput sequencing, followed by screening of differentially expressed lncRNAs and mRNAs.

Source: American Journal of Pathology - November 17, 2023 Category: Pathology Authors: Yingjie Hao, Guangduo Zhu, Lei Yu, Zhinan Ren, Weiwei Zhou, Panke Zhang, Xu Lian Tags: Regular Article Source Type: research