Opportunities and tradeoffs in single-cell transcriptomic technologies

Trends Genet. 2023 Nov 10:S0168-9525(23)00231-7. doi: 10.1016/j.tig.2023.10.003. Online ahead of print.ABSTRACTRecent technological and algorithmic advances enable single-cell transcriptomic analysis with remarkable depth and breadth. Nonetheless, a persistent challenge is the compromise between the ability to profile high numbers of cells and the achievement of full-length transcript coverage. Currently, the field is progressing and developing new and creative solutions that improve cellular throughput, gene detection sensitivity and full-length transcript capture. Furthermore, long-read sequencing approaches for single-cell transcripts are breaking frontiers that have previously blocked full transcriptome characterization. We here present a comprehensive overview of available options for single-cell transcriptome profiling, highlighting the key advantages and disadvantages of each approach.PMID:37953195 | DOI:10.1016/j.tig.2023.10.003

Source: Trends in Genetics : TIG - November 12, 2023 Category: Genetics & Stem Cells Authors: Matilde I Conte Azahara Fuentes-Trillo Cecilia Dom ínguez Conde Source Type: research