A graphitized carbon@boron carbide-mediated laccase-based amperometric biosensor for epinephrine detection

Bioelectrochemistry. 2023 Oct 21;155:108591. doi: 10.1016/j.bioelechem.2023.108591. Online ahead of print.ABSTRACTThe development of novel electron transfer mediator is important for laccase-based electrochemical biosensors. Here, graphitized carbon coated boron carbide (GC@B4C) was first used as the mediator to transfer electrons from laccase (Lac) to nickel foam (NF) electrode. The GC shell of GC@B4C particles was characterized and confirmed by HRTEM, XRD and Raman spectroscopy. The fabricated Lac/GC@B4C/NF electrode was applied for amperometric detection of epinephrine (EP). Detection results indicated Lac/GC@B4C/NF electrode has wide linear range (0.1 µM-2.6 mM), low detection limit (40 nM), high sensitivity (76.05 µA mM-1), strong anti-interference ability and favorable stability for EP detection. This work opened up the use of nonmetallic carbide as the mediator for enzyme-based electrochemical biosensors.PMID:37883859 | DOI:10.1016/j.bioelechem.2023.108591

Source: Bioelectrochemistry - October 26, 2023 Category: Biochemistry Authors: Huanyu Tang Mingyue Cui Miaorong Zhang Yan Zhang Source Type: research