Approaches and challenges in genome-wide circular RNA identification and quantification

Trends Genet. 2023 Oct 13:S0168-9525(23)00223-8. doi: 10.1016/j.tig.2023.09.006. Online ahead of print.ABSTRACTNumerous circular RNAs (circRNAs) produced from back-splicing of exon(s) have been recently revealed on a genome-wide scale across species. Although generally expressed at a low level, some relatively abundant circRNAs can play regulatory roles in various biological processes, prompting continuous profiling of circRNA in broader conditions. Over the past decade, distinct strategies have been applied in both transcriptome enrichment and bioinformatic tools for detecting and quantifying circRNAs. Understanding the scope and limitations of these strategies is crucial for the subsequent annotation and characterization of circRNAs, especially those with functional potential. Here, we provide an overview of different transcriptome enrichment, deep sequencing and computational approaches for genome-wide circRNA identification, and discuss strategies for accurate quantification and characterization of circRNA.PMID:37839990 | DOI:10.1016/j.tig.2023.09.006

Source: Trends in Genetics : TIG - October 15, 2023 Category: Genetics & Stem Cells Authors: Xu-Kai Ma Si-Nan Zhai Li Yang Source Type: research