Advances in genome-scale metabolic models of industrially important fungi

Curr Opin Biotechnol. 2023 Oct 3;84:103005. doi: 10.1016/j.copbio.2023.103005. Online ahead of print.ABSTRACTMany fungal species have been used industrially for production of biofuels and bioproducts. Developing strains with better performance in biomanufacturing contexts requires a systematic understanding of cellular metabolism. Genome-scale metabolic models (GEMs) offer a comprehensive view of interconnected pathways and a mathematical framework for downstream analysis. Recently, GEMs have been developed or updated for several industrially important fungi. Some of them incorporate enzyme constraints, enabling improved predictions of cell states and proteome allocation. Here, we provide an overview of these newly developed GEMs and computational methods that facilitate construction of enzyme-constrained GEMs and utilize flux predictions from GEMs. Furthermore, we highlight the pivotal roles of these GEMs in iterative design-build-test-learn cycles, ultimately advancing the field of fungal biomanufacturing.PMID:37797483 | DOI:10.1016/j.copbio.2023.103005
Source: Current Opinion in Biotechnology - Category: Biotechnology Authors: Source Type: research