QuantiGene Plex Represents a Promising Diagnostic Tool for Cell-of-Origin Subtyping of Diffuse Large B-Cell Lymphoma

Publication date: Available online 14 May 2015 Source:The Journal of Molecular Diagnostics Author(s): John S. Hall , Suzanne Usher , Richard J. Byers , Rebekah C. Higgins , Danish Memon , John A. Radford , Kim M. Linton Personalized management of diffuse large B-cell lymphoma (DLBCL) requires codevelopment of a companion diagnostic assay for activated B-cell and germinal center B-cell subtyping. Current classification methods are costly/complex (microarray expression profiling) or lacking in reproducibility/diagnostic precision (immunophenotyping). We investigated the potential of QuantiGene Plex (QGP)—a branched DNA signal amplification assay with high-detection sensitivity from formalin-fixed, paraffin-embedded tissue—for DLBCL subtyping. We performed in silico analysis of public DLBCL data sets to develop and validate a naïve Bayes classifier. The resulting 21-gene classifier was migrated to QGP and real-time quantitative PCR (qPCR) assays for reclassification of 40 DLBCL formalin-fixed, paraffin-embedded tumors of known subtype (20 per subtype by gene expression profiling of paired fresh-frozen tissues). Results were compared for recapitulation of microarray data and classification accuracy. The 21-gene bayesian classifier achieved mean area under the curve values >0.9 on independent validation. QGP was performed on 38 of 40 samples for 21 of 21 targets. qPCR was performed on 40 of 40 samples for 19 of 21 targets. QGP showed a higher correlation wit...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research