Formalin-Fixed, Paraffin-Embedded –Targeted Locus Capture
Chromosomal rearrangements are important drivers in cancer, and their robust detection is essential for diagnosis, prognosis, and treatment selection, particularly for bone and soft tissue tumors. Current diagnostic methods are hindered by limitations, including difficulties with multiplexing targets and poor quality of RNA. A novel targeted DNA-based next-generation sequencing method, formalin-fixed, paraffin-embedded –targeted locus capture (FFPE-TLC), has shown advantages over current diagnostic methods when applied on FFPE lymphomas, including the ability to detect novel rearrangements.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Ellen Stelloo, Ruud W.J. Meijers, Joost F. Swennenhuis, Amin Allahyar, Karima Hajo, Mario Cangiano, Wendy W.J. de Leng, Sjoerd van Helvert, Joni Van der Meulen, David Creytens, L éon C. van Kempen, Anne-Marie Cleton-Jansen, Judith V.M.G. Bovee, Wouter de Tags: Regular article Source Type: research