Small ‐scale two‐dimensional liquid chromatography for a preparative re‐purification of a highly labile tritium‐labeled compound

We report a case of a sensitive molecule that could not be isolated in pure form even though the chromatographic separation was successful. In this case, the use of a small-scale two-dimensional preparative liquid chromatography approach with a direct transfer interface to a second (tra pping) column resulted in a highly pure compound (>98% radiochemical purity). This approach combines high chromatographic resolution, accurate control over the re-purification process, minimal sample manipulation, and higher overall safety for the handling of radioactive samples.

Source: Journal of Labelled Compounds and Radiopharmaceuticals - May 15, 2023 Category: Biochemistry Authors: Martin Sandvoss, Christian Klaus, Remo Weck, Volker Derdau, Matthias Schiell Tags: RESEARCH ARTICLE Source Type: research