CRISPR-Cas9 Targeted Enrichment and Next-Generation Sequencing for Mutation Detection

Despite the rapid application of next-generation sequencing (NGS) technologies, target sequencing in regions of the genome is often required to diagnose many genetic diseases. Target enrichment can be an effective factor in reducing the cost of sequencing and the duration of sequencing. Recently, several clustered system regularly interspaced short palindromic repeats (CRISPR) –based methods (amplification-free sequencing) have been developed to target enrichment in combination with one of the NGS platforms.

Source: Journal of Molecular Diagnostics - February 23, 2023 Category: Pathology Authors: Mehrdad Malekshoar, Sajad Ataei Azimi, Arastoo Kaki, Leila Mousazadeh, Jamshid Motaei, Majid Vatankhah Tags: Review Source Type: research