CRISPR-Cas9 Targeted Enrichment and Next Generation Sequencing for Mutations Detection

Despite the rapid application of next-generation sequencing (NGS) technologies, target sequencing in regions of the genome is often required to diagnose many genetic diseases. Target enrichment can be a very effective factor in reducing the cost of sequencing and the duration of sequencing. Recently, several CRISPR-based methods (amplification-free sequencing) have been developed to target enrichment in combination with one of the NGS platforms. CRISPR-based target enrichment strategies act as an auxiliary tool to improve NGS analytical performance, thereby indirectly facilitating nucleic acid detection.

Source: Journal of Molecular Diagnostics - February 23, 2023 Category: Pathology Authors: Mehrdad Malekshoar, Sajad Ataei Azimi, Arastoo Kaki, Leila Mousazadeh, Jamshid Motaei, Majid Vatankhah Tags: Review Source Type: research