Specific detection of Treponema pallidum in clinical samples: validation of a qPCR assay combining two genomic targets

Conclusions
We validated a new qPCR for detecting the TP genome in clinical samples with excellent sensitivity and specificity. The cloning of polA and tpp47 genes for calibration would be interesting in the evaluation of bacterial loads in samples.

Source: Sexually Transmitted Infections - February 17, 2023 Category: Sexual Medicine Authors: Salle, R., Mayslich, C., Grange, P. A., Leducq, V., Ollagnier, G., Heller, U., Saule, J., Martinet, P., Robert, J.-L., Benhaddou, N., Fouere, S., Dupin, N. Tags: Original research Source Type: research