Genome-wide measurement of DNA replication fork directionality and quantification of DNA replication initiation and termination with Okazaki fragment sequencing

Nature Protocols, Published online: 18 January 2023; doi:10.1038/s41596-022-00793-5A protocol for quantitative genome-wide detection of replication initiation, fork progression and termination, based on purification and strand-specific sequencing of Okazaki fragments isolated from mammalian or yeast cells.

Source: Nature Protocols - January 18, 2023 Category: Biomedical Science Authors: Xia Wu Yaqun Liu Yves d ’Aubenton-Carafa Claude Thermes Olivier Hyrien Chun-Long Chen Nataliya Petryk Source Type: research