Fine mapping and characterization of the dominant gene SmFTSH10 conferring non-photosensitivity in eggplant (Solanum melongena L.)

Theor Appl Genet. 2022 Jun 6. doi: 10.1007/s00122-022-04078-z. Online ahead of print.ABSTRACTA candidate non photosensitive gene S m F TS H10 was identified by combining bulked segregant analysis and map‑based cloning. Low light condition often leads to poor coloration of photosensitive eggplant. Here, we obtained a non-photosensitive eggplant that can synthesize large amount of anthocyanin under shading conditions. Genetic analysis of F1 and F2 populations revealed that the phenotype of non-photosensitivity was regulated by a single dominant nuclear gene, herein temporarily designated SmFTSH10. Through Bulked segregant analysis (BSA), SNP haplotyping and fine genetic mapping delimited SmFTSH10 to a 290 kb region of eggplant chromosome 10 flanking by markers dCAPS21 and dCAPS32. Sequence analysis revealed C-base deletion in the fourth exon of SmFTSH10 resulted in premature termination of translation. The expression level of SmFTSH10 decreased significantly in anthocyanin-rich parts of mutant '145' compared with the wild-type 'LSHX'. Sequencing of 10 recombinants revealed that the C-base deletion in the 4th exon of SmFTSH10 was co-segregated with the non-photosensitive phenotype, and the sequencing analysis of the natural population of eggplant also showed that the Indel in SmFTSH10 had a high accuracy in the identification of the photosensitivity of eggplant. Light-responsive expression patterns analysis suggests that it has the same expression trend as the genes involved i...

Source: TAG. Theoretical and Applied Genetics - June 6, 2022 Category: Genetics & Stem Cells Authors: YongJun He ShaoHang Li YanXiao Dong XinTong Zhang DaLu Li Yang Liu HuoYing Chen Source Type: research