Iniciativa espanola para mejorar la transparencia en el uso de animales de experimentacion
(Source: Laboratory Animals)
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Guillen, J. Tags: News Source Type: research
Anaesthesia and analgesia in laboratory adult zebrafish: a question of refinement
This article reviews the main anaesthetic agents and protocols used in laboratory adult zebrafish, and some of the analgesic methods to be used in this species that still need more research. In addition, a systematized observation of signs is proposed to evaluate adult zebrafish welfare to reduce pain and distress. (Source: Laboratory Animals)
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Martins, T., Valentim, A. M., Pereira, N., Antunes, L. M. Tags: Special Articles Source Type: research
Severity assessment in rabbits after partial hepatectomy: Part II
Although the recognition of pain, distress and discomfort has already been described in 1985 by Morton and Griffiths there is still very little known about the establishment of score sheets especially, regarding post-surgical pain and severity assessment for laboratory animals such as rabbits. In this paper we describe the estimation of severity and recovery status of 36 female New Zealand White rabbits (NZW) in a standardized liver resection model using two different adhesive treatments and one control group. Welfare was assessed at 3–4 consecutive days after surgery using a scoring system which included the followi...
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Drude, N., Pawlowsky, K., Tanaka, H., Fukushima, K., Kögel, B., Tolba, R. H. Tags: Special Articles Source Type: research
Recommendation for severity assessment following liver resection and liver transplantation in rats: Part I
Score sheets were first introduced 30 years ago to assess pain, distress and suffering in animals. To date, however, there is still no general agreement on their use in research practice, and only a few publications can be found on this topic. In the present work, we demonstrate the use of a special score sheet for severity assessment in the first three postoperative days in two showcased studies performed on Wistar and Lewis rats undergoing liver resection or orthotopic liver transplantation, respectively. Scoring of different criteria and the total score were evaluated within each intervention. Additionally, both procedu...
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Kanzler, S., Rix, A., Czigany, Z., Tanaka, H., Fukushima, K., Kögel, B., Pawlowsky, K., Tolba, R. H. Tags: Special Articles Source Type: research
Morbidity scoring after abdominal surgery
Postoperative monitoring of pain and distress in small rodents is not standardized, and widely accepted score sheets are not available. Here we describe a score sheet used in abdominal surgery of rodents, with particular reference to procedures involving the liver. (Source: Laboratory Animals)
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Graf, R., Cinelli, P., Arras, M. Tags: Special Articles Source Type: research
Severity assessment and scoring for neurosurgical models in rodents
The most important acute neurological diseases seen at neurosurgery departments are traumatic brain injuries (TBI) and subarachnoid hemorrhages (SAH). In both diseases the pathophysiological sequela are complex and have not been fully understood up to now, and rodent models using rats and mice are most suitable for the investigation of the pathophysiological details. In both models, surgery is performed under anesthesia, followed by assessment of their functional outcome and behavioral testing before brain tissue analysis after euthanasia. Postoperative analgesia is mandatory, and supplementary care is highly recommended f...
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Pinkernell, S., Becker, K., Lindauer, U. Tags: Special Articles Source Type: research
Osteotomy models - the current status on pain scoring and management in small rodents
Fracture healing is a complex regeneration process which produces new bone tissue without scar formation. However, fracture healing disorders occur in approximately 10% of human patients and cause severe pain and reduced quality of life. Recently, the development of more standardized, sophisticated and commercially available osteosynthesis techniques reflecting clinical approaches has increased the use of small rodents such as rats and mice in bone healing research dramatically. Nevertheless, there is no standard for pain assessment, especially in these species, and consequently limited information regarding the welfare as...
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Lang, A., Schulz, A., Ellinghaus, A., Schmidt-Bleek, K. Tags: Special Articles Source Type: research
The more the merrier? Scoring, statistics and animal welfare in experimental autoimmune encephalomyelitis
Experimental autoimmune encephalomyelitis (EAE) is a frequently used animal model for the investigation of autoimmune processes in the central nervous system. As such, EAE is useful for modelling certain aspects of multiple sclerosis, a human autoimmune disease that leads to demyelination and axonal destruction. It is an important tool for investigating pathobiology, identifying drug targets and testing drug candidates. Even though EAE is routinely used in many laboratories and is often part of the routine assessment of knockouts and transgenes, scoring of the disease course has not become standardized in the community, wi...
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Palle, P., Ferreira, F. M., Methner, A., Buch, T. Tags: Special Articles Source Type: research
Analgesia in clinically relevant rodent models of sepsis
Postoperative analgesia in rodent sepsis models has been considerably neglected in the past. However, intentions to model clinical practice, increasing awareness of animal ethics, efforts to apply the 3Rs (replacement, reduction, refinement), and stricter legislation argue for a change in this respect. In this review, we describe different concepts of analgesia in rodent models of sepsis focusing on opioid agonists as well as non-opioid analgesics. Advantages and pitfalls in study design and side-effects are discussed. Score sheets should be used to adapt analgesia or to terminate experiments using humane endpoints. Furthe...
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Jeger, V., Hauffe, T., Nicholls-Vuille, F., Bettex, D., Rudiger, A. Tags: Special Articles Source Type: research
What the literature tells us about score sheet design
Score sheets are an essential tool of animal welfare. They allow transparent assessments to be made of animal health and behavior during animal experiments and they define interventions when deviations from normal status are detected. As such, score sheets help to refine animal experiments as part of the 3R (replacement, reduction and refinement) concept. This mini review aims at summarizing the scarce literature available on score sheet design. (Source: Laboratory Animals)
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Bugnon, P., Heimann, M., Thallmair, M. Tags: Special Articles Source Type: research
Score sheets and analgesia
(Source: Laboratory Animals)
Source: Laboratory Animals - November 30, 2016 Category: Research Authors: Golledge, H., Jirkof, P. Tags: Special Editorial Source Type: research
Thanks to Reviewers
(Source: Laboratory Animals)
Source: Laboratory Animals - November 30, 2016 Category: Research Tags: Thanks to Reviewers Source Type: research
Recomendaciones para controles microbiologicos ambientales y de agua en un animalario
(Source: Laboratory Animals)
Source: Laboratory Animals - September 25, 2016 Category: Research Authors: Parra, L. Tags: News Source Type: research
15e Symposium ComTech
(Source: Laboratory Animals)
Source: Laboratory Animals - September 25, 2016 Category: Research Tags: News Source Type: research
Case Reports for Laboratory Animals
(Source: Laboratory Animals)
Source: Laboratory Animals - September 25, 2016 Category: Research Authors: Bomzon, A. Tags: News Source Type: research
