Filtered By:
Source: J Biochem Mol Biol
Vaccination: Hepatitis Vaccine
This page shows you your search results in order of date.
Order by Relevance | Date
Total 617 results found since Jan 2013.
Overexpression and purification of PreS region of hepatitis B virus antigenic surface protein adr subtype in Escherichia coli
This report deals with overexpression and purification of adr subtype of surface antigen that is more prevalent in Pakistan. PreS region, comprising 119 aa preS1 region plus a 55 aa preS2 region plus 11 aa from the N-terminal S region, was inserted in pET21a+ vector, cloned in E. coli DH5alpha cells and expressed in E. coli BL21 codon+ cells. The conditions for over expression were optimized using different concentrations of IPTG (0.01-5 mM), and incubating the cells at different temperatures (23-41 degrees C) for different durations (0-6 h). The cells were grown under the given optimized conditions (0.5 mM IPTG concentrat...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Naaz Abbas Aftab Ahmad Abdul Rauf Shakoori Source Type: research
