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Source: J Biochem Mol Biol
Infectious Disease: Gastroenteritis
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Total 3717 results found since Jan 2013.
Cloning and characterization of a single chain antibody to glucose oxidase from a murine hybridoma
We describe here the generation of a panel of monoclonal antibodies to native and heat inactivated GOD. One of the hybridomas, E13BC8, was used for cloning of a single chain antibody (scFv). This scFv was expressed in Escherichia coli XL1-blue with the help of the vector system pOPE101. The scFv was isolated from the periplasmic fraction and detected by western blotting. It reacts specifically with soluble active GOD but does not recognize denatured GOD adsorbed to the solid phase. The same binding properties were also found for the monoclonal antibody E13BC8.PMID:18047781 | DOI:10.5483/bmbrep.2007.40.6.875
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Frank Sellrie J örg A Schenk Olaf Behrsing Oliver Drechsel Burkhard Micheel Source Type: research
Characterization of uridine-diphosphate dependent flavonoid glucosyltransferase from Oryza sativa
J Biochem Mol Biol. 2007 Nov 30;40(6):870-4. doi: 10.5483/bmbrep.2007.40.6.870.ABSTRACTWe cloned a uridine-diphosphate dependent glycosyltransferase RUGT-10 from Oryza sativa. The recombinant enzyme was expressed by glutathione-S transferase gene fusion system in Escherichia coli. RUGT10 showed different regioselectivity depending on the structures of substrates (e.g. flavanone, flavonol, and flavone). Apparently, flavanone such as naringenin and eriodictyol gave one 7-O-glucoside while flavone and flavonol gave more than two products with preferential glucosylation position of hydroxyl group at C-3 position.PMID:18047780 ...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Byoung Seok Hong Jeong Ho Kim Na Yeon Kim Bong-Gyu Kim Youhoon Chong Joong-Hoon Ahn Source Type: research
Molecular cloning and functional analysis of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from hazel (Corylus avellana L. Gasaway)
J Biochem Mol Biol. 2007 Nov 30;40(6):861-9. doi: 10.5483/bmbrep.2007.40.6.861.ABSTRACTThe enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR (designated as CgHMGR, GenBank accession number EF206343) from hazel (Corylus avellana L. Gasaway), a taxol-producing plant species. The full-length cDNA of CgHMGR was 2064 bp containing a 1704-bp ORF encoding 567 amino acids. Bioinformatic analyses revealed that the deduced CgHMGR had ext...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Yechun Wang Binhui Guo Fei Zhang Hongyan Yao Zhiqi Miao Kexuan Tang Source Type: research
Evidence of tandem repeat and extra thiol-groups resulted in the polymeric formation of bovine haptoglobin: a unique structure of Hp 2-2 phenotype
In this study, we expressed entire bovine Hp in E. coli as a alphabeta linear form. Interestingly, the antibodies prepared against this form could recognize the subunit of native Hp. In stead of a complicated column method, the antibody was able to isolate bovine Hp via immunoaffinity and gel-filtration columns. The isolated Hp is polymeric containing two major molecular forms (660 and 730 kDa). Their size and hemoglobin binding complex are significantly larger than that of human Hp 2-2. The amino-acid sequence deducted from the nucleotide sequence is similar to human Hp 2 containing a tandem repeat over the alpha chain. T...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Yi An Lai I Hsiang Lai Chi Feng Tseng James Lee Simon J T Mao Source Type: research
Construction and expression of mutant cDNAs responsible for genetic polymorphism in aldehyde oxidase in Donryu strain rats
In this study, we constructed an E. coli expression system of four types of AO cDNA including Mut-1 with (377G, 2604T) and Mut-2 with (377A, 2604C) as well as naturally existing nucleotide sequences of UM and PM in order to clarify which one is responsible for the polymorphism. Mut-1 and Mut-2 showed almost the same high and low activity as that of the UM and PM groups, respectively. Thus, the expression study of mutant AO cDNA directly revealed that the nucleotide substitution of 377G > A, but not that of 2604C > T, will play a critical role in the genetic polymorphism of AO in Donryu strain rats. The reason amino a...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Mayuko Adachi Kunio Itoh Akiko Masubuchi Nobuaki Watanabe Yorihisa Tanaka Source Type: research
Overexpression and purification of PreS region of hepatitis B virus antigenic surface protein adr subtype in Escherichia coli
This report deals with overexpression and purification of adr subtype of surface antigen that is more prevalent in Pakistan. PreS region, comprising 119 aa preS1 region plus a 55 aa preS2 region plus 11 aa from the N-terminal S region, was inserted in pET21a+ vector, cloned in E. coli DH5alpha cells and expressed in E. coli BL21 codon+ cells. The conditions for over expression were optimized using different concentrations of IPTG (0.01-5 mM), and incubating the cells at different temperatures (23-41 degrees C) for different durations (0-6 h). The cells were grown under the given optimized conditions (0.5 mM IPTG concentrat...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Naaz Abbas Aftab Ahmad Abdul Rauf Shakoori Source Type: research
Cloning and characterization of a single chain antibody to glucose oxidase from a murine hybridoma
We describe here the generation of a panel of monoclonal antibodies to native and heat inactivated GOD. One of the hybridomas, E13BC8, was used for cloning of a single chain antibody (scFv). This scFv was expressed in Escherichia coli XL1-blue with the help of the vector system pOPE101. The scFv was isolated from the periplasmic fraction and detected by western blotting. It reacts specifically with soluble active GOD but does not recognize denatured GOD adsorbed to the solid phase. The same binding properties were also found for the monoclonal antibody E13BC8.PMID:18047781 | DOI:10.5483/bmbrep.2007.40.6.875
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Frank Sellrie J örg A Schenk Olaf Behrsing Oliver Drechsel Burkhard Micheel Source Type: research
Characterization of uridine-diphosphate dependent flavonoid glucosyltransferase from Oryza sativa
J Biochem Mol Biol. 2007 Nov 30;40(6):870-4. doi: 10.5483/bmbrep.2007.40.6.870.ABSTRACTWe cloned a uridine-diphosphate dependent glycosyltransferase RUGT-10 from Oryza sativa. The recombinant enzyme was expressed by glutathione-S transferase gene fusion system in Escherichia coli. RUGT10 showed different regioselectivity depending on the structures of substrates (e.g. flavanone, flavonol, and flavone). Apparently, flavanone such as naringenin and eriodictyol gave one 7-O-glucoside while flavone and flavonol gave more than two products with preferential glucosylation position of hydroxyl group at C-3 position.PMID:18047780 ...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Byoung Seok Hong Jeong Ho Kim Na Yeon Kim Bong-Gyu Kim Youhoon Chong Joong-Hoon Ahn Source Type: research
Molecular cloning and functional analysis of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from hazel (Corylus avellana L. Gasaway)
J Biochem Mol Biol. 2007 Nov 30;40(6):861-9. doi: 10.5483/bmbrep.2007.40.6.861.ABSTRACTThe enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR (designated as CgHMGR, GenBank accession number EF206343) from hazel (Corylus avellana L. Gasaway), a taxol-producing plant species. The full-length cDNA of CgHMGR was 2064 bp containing a 1704-bp ORF encoding 567 amino acids. Bioinformatic analyses revealed that the deduced CgHMGR had ext...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Yechun Wang Binhui Guo Fei Zhang Hongyan Yao Zhiqi Miao Kexuan Tang Source Type: research
Evidence of tandem repeat and extra thiol-groups resulted in the polymeric formation of bovine haptoglobin: a unique structure of Hp 2-2 phenotype
In this study, we expressed entire bovine Hp in E. coli as a alphabeta linear form. Interestingly, the antibodies prepared against this form could recognize the subunit of native Hp. In stead of a complicated column method, the antibody was able to isolate bovine Hp via immunoaffinity and gel-filtration columns. The isolated Hp is polymeric containing two major molecular forms (660 and 730 kDa). Their size and hemoglobin binding complex are significantly larger than that of human Hp 2-2. The amino-acid sequence deducted from the nucleotide sequence is similar to human Hp 2 containing a tandem repeat over the alpha chain. T...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Yi An Lai I Hsiang Lai Chi Feng Tseng James Lee Simon J T Mao Source Type: research
Construction and expression of mutant cDNAs responsible for genetic polymorphism in aldehyde oxidase in Donryu strain rats
In this study, we constructed an E. coli expression system of four types of AO cDNA including Mut-1 with (377G, 2604T) and Mut-2 with (377A, 2604C) as well as naturally existing nucleotide sequences of UM and PM in order to clarify which one is responsible for the polymorphism. Mut-1 and Mut-2 showed almost the same high and low activity as that of the UM and PM groups, respectively. Thus, the expression study of mutant AO cDNA directly revealed that the nucleotide substitution of 377G > A, but not that of 2604C > T, will play a critical role in the genetic polymorphism of AO in Donryu strain rats. The reason amino a...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Mayuko Adachi Kunio Itoh Akiko Masubuchi Nobuaki Watanabe Yorihisa Tanaka Source Type: research
Overexpression and purification of PreS region of hepatitis B virus antigenic surface protein adr subtype in Escherichia coli
This report deals with overexpression and purification of adr subtype of surface antigen that is more prevalent in Pakistan. PreS region, comprising 119 aa preS1 region plus a 55 aa preS2 region plus 11 aa from the N-terminal S region, was inserted in pET21a+ vector, cloned in E. coli DH5alpha cells and expressed in E. coli BL21 codon+ cells. The conditions for over expression were optimized using different concentrations of IPTG (0.01-5 mM), and incubating the cells at different temperatures (23-41 degrees C) for different durations (0-6 h). The cells were grown under the given optimized conditions (0.5 mM IPTG concentrat...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Naaz Abbas Aftab Ahmad Abdul Rauf Shakoori Source Type: research
Evidence of tandem repeat and extra thiol-groups resulted in the polymeric formation of bovine haptoglobin: a unique structure of Hp 2-2 phenotype
In this study, we expressed entire bovine Hp in E. coli as a alphabeta linear form. Interestingly, the antibodies prepared against this form could recognize the subunit of native Hp. In stead of a complicated column method, the antibody was able to isolate bovine Hp via immunoaffinity and gel-filtration columns. The isolated Hp is polymeric containing two major molecular forms (660 and 730 kDa). Their size and hemoglobin binding complex are significantly larger than that of human Hp 2-2. The amino-acid sequence deducted from the nucleotide sequence is similar to human Hp 2 containing a tandem repeat over the alpha chain. T...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Yi An Lai I Hsiang Lai Chi Feng Tseng James Lee Simon J T Mao Source Type: research
Construction and expression of mutant cDNAs responsible for genetic polymorphism in aldehyde oxidase in Donryu strain rats
In this study, we constructed an E. coli expression system of four types of AO cDNA including Mut-1 with (377G, 2604T) and Mut-2 with (377A, 2604C) as well as naturally existing nucleotide sequences of UM and PM in order to clarify which one is responsible for the polymorphism. Mut-1 and Mut-2 showed almost the same high and low activity as that of the UM and PM groups, respectively. Thus, the expression study of mutant AO cDNA directly revealed that the nucleotide substitution of 377G > A, but not that of 2604C > T, will play a critical role in the genetic polymorphism of AO in Donryu strain rats. The reason amino a...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Mayuko Adachi Kunio Itoh Akiko Masubuchi Nobuaki Watanabe Yorihisa Tanaka Source Type: research
Overexpression and purification of PreS region of hepatitis B virus antigenic surface protein adr subtype in Escherichia coli
This report deals with overexpression and purification of adr subtype of surface antigen that is more prevalent in Pakistan. PreS region, comprising 119 aa preS1 region plus a 55 aa preS2 region plus 11 aa from the N-terminal S region, was inserted in pET21a+ vector, cloned in E. coli DH5alpha cells and expressed in E. coli BL21 codon+ cells. The conditions for over expression were optimized using different concentrations of IPTG (0.01-5 mM), and incubating the cells at different temperatures (23-41 degrees C) for different durations (0-6 h). The cells were grown under the given optimized conditions (0.5 mM IPTG concentrat...
Source: J Biochem Mol Biol - December 1, 2007 Category: Molecular Biology Authors: Naaz Abbas Aftab Ahmad Abdul Rauf Shakoori Source Type: research
