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Expression of mouse small interfering RNAs in lettuce using artificial microRNA technology.
Abstract Artificial miRNA technology enables the generation of siRNAs to regulate the expression of targeted genes. However, the application of siRNAs to alter gene expression is challenging due to their instability and requires a means to efficiently deliver siRNAs into the host. Here, we report that the siRNAs targeted to animal mRNAs can be heterologously expressed and stably produced in lettuce. We have modified rice miRNA precursors to produce siRNAs in lettuce with the potential to target mRNAs of mouse complement 3 (C3) and coagulation factor 7 (CF7). Expression of primary and mature siRNAs in the transge...
Source: BioTechniques - January 14, 2020 Category: Biotechnology Authors: Kakeshpour T, Tamang TM, Park WD, Manohar M, Yang J, Hirschi KD, Park S Tags: Biotechniques Source Type: research