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Design and Validation of small interfering RNA on Respiratory Syncytial Virus M2-2 gene: a potential approach in RNA interference on viral replication.
In conclusion, this study showed the effectiveness of siRNA in silencing M2-2 gene both at the protein and mRNA level which could potentially be used as a novel therapeutic agent in the treatment of RSV infection. However, further study is warranted to investigate the silencing effect of M2-2 protein and inhibition of RSV infection. PMID: 27432115 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - July 14, 2016 Category: Virology Authors: Kin CV, Atika Aziz NA, Hudu SA, Harmal NS, Syahrilnizam A, Jalilian FA, Zamberi S Tags: J Virol Methods Source Type: research

In-vitro inhibition of spring viremia of carp virus replication by RNA interference targeting the RNA-dependent RNA polymerase gene.
Abstract Spring viremia of carp, a fatal viral disease, is caused by the spring viremia of carp virus (SVCV) and can result in up to 70% mortalities in common carps and significant economic losses in several other cyprinid aquaculture. The present study aimed to investigate the possible control of SVCV replication in Epithelioma papulosum cyprini (EPC) cells using the RNA interference technology targeting the RNA-dependent RNA polymerase (L) gene of the SVCV that is essential for its replication. Three stealth small interfering RNA (siRNA) sequences were designed to target three different regions on the SVCV-L gen...
Source: Journal of Virological Methods - October 15, 2018 Category: Virology Authors: Marzouk Fouad A, Soliman H, Abdallah ESH, Ibrahim S, El-Matbouli M, Elkamel AA Tags: J Virol Methods Source Type: research

Development of transgenic okra (Abelmoschus esculentus L. Moench) lines having RNA mediated resistance to Yellow vein mosaic virus (Geminiviridae)
J Virol Methods. 2022 Jan 6;301:114457. doi: 10.1016/j.jviromet.2022.114457. Online ahead of print.ABSTRACTBegomovirus Yellow vein mosaic virus causes severe yield losses in okra and even the resistant lines developed through conventional breeding show susceptibility at various levels. This paper describes the development of YVMV resistant lines through RNAi strategy. A universal ihpRNA construct harbouring βC1 ORF from the β-satellite of the begomovirus was designed using pRNAi-LIC plasmid. Complementarity checks in sequence databases had shown no off-target effects by the target region and the success of siRNA in inter...
Source: Journal of Virological Methods - January 9, 2022 Category: Virology Authors: Kelkar Vipul Ganesh Deepu Mathew Rehna Augustine Soni K B Swapna Alex Shylaja M R Anita Cherian K Source Type: research

In Vitro Inhibition of Cyprinid Herpesvirus-3 Replication by RNAi.
Abstract Cyprinid herpesvirus-3 (CyHV-3) is an aetiological agent of a notifiable disease that causes high mortality rates affecting both the common and koi carp Cyprinus carpio L. There is no current treatment strategy to save CyHV-3 infected fish. RNA mediated interference (RNAi) is an emerging strategy used for understanding gene function and is a promising method in developing novel therapeutics and antiviral medications. For this study, the possibility of activating the RNAi pathway by the use of small interfering (si)RNAs was tested to inhibit in vitro viral replication of CyHV-3 in common carp brain (CCB) c...
Source: Journal of Virological Methods - May 31, 2014 Category: Virology Authors: Gotesman M, Soliman H, Besch R, El-Matbouli M Tags: J Virol Methods Source Type: research

Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection.
Abstract RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3'UTR were established. Certain cell lines showed an effective, sequence-specific amiR-mediated silencing activity that was accomplished by degradation of the target mRNA, as demonstrated in...
Source: Journal of Virological Methods - January 6, 2014 Category: Virology Authors: Gismondi MI, Ortiz XP, Currá AP, Asurmendi S, Taboga O Tags: J Virol Methods Source Type: research

Combined knockdown of RL13 and UL128 for release of cell-free infectivity from recent HCMV isolates
J Virol Methods. 2022 May 5:114537. doi: 10.1016/j.jviromet.2022.114537. Online ahead of print.ABSTRACTDue to strictly cell-associated growth, experiments requiring cell-free virus are not applicable to recent clinical HCMV isolates to date. On the other hand, adaptation to cell-free growth is associated with undesirable changes in the viral gene regions RL13 and UL128. We had previously found that siRNA-mediated reduction of UL128 allowed transient release of cell-free virus by clinical isolates, and now hypothesized that virus yield could be further increased by additional knockdown of RL13. Despite the extensive polymor...
Source: Journal of Virological Methods - May 8, 2022 Category: Virology Authors: Nina Weiler Kerstin Laib Sampaio Richard James Stanton Christian Sinzger Source Type: research