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Source: Insect Biochemistry and Molecular Biology

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Total 19 results found since Jan 2013.

Masculinizer is not post-transcriptionally regulated by female-specific piRNAs during sex determination in the Asian corn borer, Ostrinia furnacalis
Insect Biochem Mol Biol. 2023 Apr 17:103946. doi: 10.1016/j.ibmb.2023.103946. Online ahead of print.ABSTRACTLepidopteran insects are heterogametic in females, although most insect species are heterogametic in males. In a lepidopteran model species, the silkworm Bombyx mori (Bombycoidea), the uppermost sex determinant Feminizer (Fem) has been identified on the female-specific W chromosome. Fem is a precursor of PIWI-interacting small RNA (piRNA). Fem piRNA forms a complex with Siwi, one of the two B. mori PIWI-clade Argonaute proteins. In female embryos, Fem piRNA-Siwi complex cleaves the mRNA of the male-determining gene M...
Source: Insect Biochemistry and Molecular Biology - April 17, 2023 Category: Biochemistry Authors: Takahiro Fukui Keisuke Shoji Takashi Kiuchi Yutaka Suzuki Susumu Katsuma Source Type: research

Both LmDicer-1 and two LmDicer-2s participate in siRNA-mediated RNAi pathway and contribute to high gene silencing efficiency in Locusta migratoria
In this study, we identified one LmDicer-1 and two LmDicer-2 (LmDicer-2a and LmDicer-2b) genes in Locusta migratoria. The RNAi of RNAi assay showed that knockdown of each of the Dicer genes reduced RNAi efficiency against a target gene (Lmβ-Tubulin), suggesting that all these genes participated in the siRNA-mediated RNAi pathway. Sequence analyses of the siRNAs generated from dsLmβ-Tubulin after silencing each LmDicer gene showed no significant difference in the pattern of siRNAs mapped to dsLmβ-Tubulin. This result indicated that all the three LmDicers are capable of generating siRNAs from the dsRNA. We then generated ...
Source: Insect Biochemistry and Molecular Biology - November 6, 2022 Category: Biochemistry Authors: Lu Gao Yanli Wang Mureed Abbas Tingting Zhang Enbo Ma Hans Merzendorfer Kun Yan Zhu Jianzhen Zhang Source Type: research

The stability and sequence cleavage preference of dsRNA are key factors differentiating RNAi efficiency between migratory locust and Asian corn borer
Insect Biochem Mol Biol. 2022 Feb 5:103738. doi: 10.1016/j.ibmb.2022.103738. Online ahead of print.ABSTRACTWe compared the stability of double-stranded RNA (dsRNA) in each of two body fluids (hemolymph, midgut fluid) and in each of two tissues (integument, midgut), and the uptake of dsRNA in each of two cultured tissues (integument, midgut) between the migratory locust (Locusta migratoria) and the Asian corn borer (Ostrinia furnacalis). We further compared the abundance of putative small interfering RNAs (siRNAs) generated from each of two dsRNAs (dsβ-actin, dsEf1α) and the preference of dsRNA cleavages between the two i...
Source: Insect Biochemistry and Molecular Biology - February 8, 2022 Category: Biochemistry Authors: Yunhe Fan Huifang Song Mureed Abbas Yanli Wang Xiaojian Liu Tao Li Enbo Ma Kun Yan Zhu Jianzhen Zhang Source Type: research

Insights into gene manipulation techniques for Acari functional genomics
Insect Biochem Mol Biol. 2022 Feb 5:103705. doi: 10.1016/j.ibmb.2021.103705. Online ahead of print.ABSTRACTFunctional genomics is an essential tool for elucidating the structure and function of genes in any living organism. Here, we review the use of different gene manipulation techniques in functional genomics of Acari (mites and ticks). Some of these Acari species inflict severe economic losses to managed crops and health problems to humans, wild and domestic animals, but many also provide important ecosystem services worldwide. Currently, RNA interference (RNAi) is the leading gene expression manipulation tool followed ...
Source: Insect Biochemistry and Molecular Biology - February 8, 2022 Category: Biochemistry Authors: Beatrice T Nganso G Pines V Soroker Source Type: research

The stability and sequence cleavage preference of dsRNA are key factors differentiating RNAi efficiency between migratory locust and Asian corn borer
Insect Biochem Mol Biol. 2022 Feb 5;143:103738. doi: 10.1016/j.ibmb.2022.103738. Online ahead of print.ABSTRACTWe compared the stability of double-stranded RNA (dsRNA) in each of two body fluids (hemolymph, midgut fluid) and in each of two tissues (integument, midgut), and the uptake of dsRNA in each of two cultured tissues (integument, midgut) between the migratory locust (Locusta migratoria) and the Asian corn borer (Ostrinia furnacalis). We further compared the abundance of putative small interfering RNAs (siRNAs) generated from each of two dsRNAs (dsβ-actin, dsEf1α) and the preference of dsRNA cleavages between the t...
Source: Insect Biochemistry and Molecular Biology - February 8, 2022 Category: Biochemistry Authors: Yunhe Fan Huifang Song Mureed Abbas Yanli Wang Xiaojian Liu Tao Li Enbo Ma Kun Yan Zhu Jianzhen Zhang Source Type: research

Roles for Drosophila cap1 2'-O-ribose methyltransferase in the small RNA silencing pathway associated with Argonaute 2.
Abstract Cap1 2'-O-ribose methyltransferase (CMTR1) modifies RNA transcripts containing the 7-methylguanosine cap via 2'-O-ribose methylation of the first transcribed nucleotide, yielding cap1 structures. However, the role of CMTR1 in small RNA-mediated gene silencing remains unknown. Here, we identified and characterized a Drosophila CMTR1 gene (dCMTR1) mutation. We found that the catalytic activity of dCMTR1 was involved in the biogenesis of small interfering RNAs (siRNAs) but not microRNAs. Additionally, dCMTR1 interacted with R2D2, a key component for the assembly of the RNA-induced silencing complex (RISC) co...
Source: Insect Biochemistry and Molecular Biology - June 2, 2020 Category: Biochemistry Authors: Lee S, Hong JS, Lim DH, Lee YS Tags: Insect Biochem Mol Biol Source Type: research

Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells.
Abstract The potential utility of RNA interference (RNAi) to control insect pests and viral infections depends largely on the target organism's ability to systemically spread the RNAi response. The efficacy of systemic RNAi varies among insects, though it has been shown to be high in the red flour beetle, Tribolium castaneum. We identified an extracellular RNAi signal that is present in the culture medium of T. castaneum (TcA) cells after treatment with long dsRNA specific for a luciferase reporter gene. Luciferase-specific siRNAs were detected in extracellular vesicles (EVs) that were purified from the culture me...
Source: Insect Biochemistry and Molecular Biology - April 13, 2020 Category: Biochemistry Authors: Mingels L, Wynant N, Santos D, Peeters P, Gansemans Y, Billen J, Van Nieuwerburgh F, Broeck JV Tags: Insect Biochem Mol Biol Source Type: research

Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti.
This study lays critical groundwork for pursuing ZIKV transmission-blocking strategies that exploit the Ae. aegypti exo-siRNA response for arbovirus suppression in natural populations. PMID: 31103782 [PubMed - as supplied by publisher]
Source: Insect Biochemistry and Molecular Biology - May 15, 2019 Category: Biochemistry Authors: Magalhaes T, Bergren NA, Bennett SL, Borland EM, Hartman DA, Lymperopoulos K, Sayre R, Borlee BR, Campbell CL, Foy BD, Olson KE, Blair CD, Black W, Kading RC Tags: Insect Biochem Mol Biol Source Type: research

Small RNA responses of Culex mosquitoes and cell lines during acute and persistent virus infection.
In conclusion, Hsu cells generated Merida virus-derived piRNAs, but our data suggests that the major sRNA response of Culex cells and mosquitoes to WNV infection is the exogenous siRNA response. It is also evident that sRNA responses differ significantly between specific virus-mosquito combinations. Future work using additional Culex-borne viruses may further elucidate how virus-derived piRNAs are generated in Culex cells and what role they may play in controlling replication of different viruses. PMID: 30959110 [PubMed - as supplied by publisher]
Source: Insect Biochemistry and Molecular Biology - April 4, 2019 Category: Biochemistry Authors: Rückert C, Prasad AN, Garcia-Luna SM, Robison A, Grubaugh ND, Weger-Lucarelli J, Ebel GD Tags: Insect Biochem Mol Biol Source Type: research

Small interfering RNA pathway contributes to antiviral immunity in Spodoptera frugiperda (Sf9) cells following Autographa californica multiple nucleopolyhedrovirus infection.
Abstract Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a well-known virus in the Baculoviridae family. Presence of the p35 gene in the AcMNPV genome as a suppressor of the short interfering RNA (siRNA) pathway is a strong reason for the importance of the siRNA pathway in the host cellular defense. Given that, here we explored the roles of Dicer-2 (Dcr2) and Argonaute 2 (Ago2) genes, key factors in the siRNA pathway in response to AcMNPV infection in Spodoptera frugiperda Sf9 cells. The results showed that the transcript levels of Dcr2 and Ago2 increased in response to AcMNPV infection particular...
Source: Insect Biochemistry and Molecular Biology - July 31, 2018 Category: Biochemistry Authors: Karamipour N, Fathipour Y, Talebi AA, Asgari S, Mehrabadi M Tags: Insect Biochem Mol Biol Source Type: research

The role of a single gene encoding the Single von Willebrand factor C-domain protein (SVC) in bumblebee immunity extends beyond antiviral defense.
Abstract The Single von Willebrand factor C-domain proteins (SVCs) are a group of short proteins mainly found in arthropods. They are proposed to be responsive in relation to environmental challenges including the nutritional status, bacterial and viral infections. The SVC protein Vago acts as a cross-talk molecule between the small interfering RNA (siRNA) pathway and the Jak/STAT pathway upon viral infection in Drosophila melanogaster and Culex mosquito cells. Unlike flies and mosquitoes that possess diverse SVCs, most bee species only have one of which the function remains unclear. Here we investigated whether t...
Source: Insect Biochemistry and Molecular Biology - October 23, 2017 Category: Biochemistry Authors: Wang H, Smagghe G, Meeus I Tags: Insect Biochem Mol Biol Source Type: research

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